Table 1.
Primer | Sequence (5′ to 3′) | |
---|---|---|
GAPDH | F | GAAGGTGAAGGTCGGAGTC |
R | GAAGATGGTGATGGGATTTC | |
IL-6 | F | AAATTCGGTACATCCTCGACGG |
R | GGAAGGTTCAGGTTGTTTTCTGC | |
TNFα | F | AACCTCCTCTCTGCCATCAA |
R | CCAAAGTAGACCTGCCCAGA | |
IL-1β | F | TTCCATTTTGTTTGCCTTAT |
R | TTATGGGAAAGTCTCAAAAC | |
TIMP1 | F | TTATCCATCCCCTGCAAACTG |
R | CAAGGTGACGGGACTGGAA | |
αSMA | F | GCGTGGCTATTCCTTCGTTACT |
R | CCGATGAAGGATGGCTGGAACA | |
ProCollagen I | F | CAAGAGGAAGGCCAAGTCGAG |
R | CGTTGTCGCAGACGCAGAT | |
TGFβ1 | F | TGACAGCAGGGATAACACACT |
R | CGCACGCAGCAGTTCTTCTCC |
Abbreviations: GAPDH Glyceraldehyde 3-phosphate dehydrogenase; IL-6 Interleukin 6; TNFα Tumour necrosis factor alpha; IL-1β Interleukin 1 beta; TIMP1 Tissue inhibitor of metalloproteinases 1; αSMA Smooth muscle actin alpha; TGFβ Transforming growth factor beta.