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. 2022 Jul 11;14(14):3361. doi: 10.3390/cancers14143361

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

SNS-032 effect on cell growth and cell death by IncuCyte Zoom Live-Imaging analysis. (A) live phase-contrast images were taken using the IncuCyte Zoom Live-Imaging system to determine the effect of SNS-032 (0.1 and 0.2 μM) on cell growth over a 72 h period (n = 3). Scale bar of representative images = 200 μm. (B) Red fluorescence dead cell marker ethidium homodimer-1 was added to inhibitor-treated cells to determine the effect of SNS-032 or palbociclib (0.1 μM) on cell death over a 72 h period (n = 3). Scale bar of representative images = 100 μm. Significant restrictions in cellular growth and increases in cell death were shown in TNBC cell lines with SNS-032 treatment. All p-values were reported with the following associated symbols: p < 0.05 (*), and all tests were two-sided.