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. 2022 Jul 16;11(14):2213. doi: 10.3390/cells11142213

Figure 8.

Figure 8

FCCP induces premature senescence. Primary non-senescent HUVEC were left untreated (control, ctrl) or treated with 5 µM of FCCP for 6 days. (A) Senescence-associated-β-galactosidase (SA-β-gal) staining. Scale bar = 200 µm. (B) Quantification of SA-β-gal-positive cells (n = 4, * p < 0.05, paired Student’s t-test). (C,D) Representative western blots of senescence markers p16 and p21 (upper panels) and densitometric quantification of p16 and p21 after normalization to vinculin (lower panels (n = 4, * p < 0.05, paired Student’s t-test). All data represent mean + SD.