Table 5.
Objective | Method | Ref. |
---|---|---|
RNA or DNA quantification | UV-Vis spectrophotometry | [93,96,97] |
Fluorescence spectrophotometry | [79] | |
Target-specific fluorescence detection | [93,96] | |
Capillary electrophoresis separation of fluorescently labelled nucleic acids | [93,96] | |
qRT-PCR | [97,98,99] | |
Gel electrophoresis on 1% agarose gel | [87] | |
miRNA expression profiles | Provides 100% coverage of the miRNAs in the miRBase database | [96] |
qRT-PCR—Real-time polymerase chain reaction.