Table 6.
Preparation methods of nanoparticles for genetic material delivery.
| Nanoparticle | Method | CPPs | Ref. |
|---|---|---|---|
| Gold nanoparticles | Layer-by-layer | Stirring speed and time | [8,106] |
| Polyelectrolyte concentration | |||
| Laser ablation in liquid | Stirring speed and time | [107] | |
| Ultracentrifugation speed and time | |||
| Liposomes | Film dispersion method | Incubation time, temperature | [79] |
| Thin film hydration method | Evaporation time, pressure, temperature | [38,108,109,110,111,112,113,114,115] | |
| Hydration time, temperature | |||
| Ethanol injection method | Injection rate | [116] | |
| Polymeric nanoparticles | o/w single emulsion method | Mixing speed, temperature | [77,78] |
| Double-emulsion method | Sonication time, amplitude | [76] | |
| Stirring time, temperature | |||
| SLN | Solvent diffusion method | Sonication time | [80,94] |
| Agitation time, temperature, speed | |||
| Film-ultrasonic method | Sonication time | [95] |
SLN—solid lipid nanoparticle; o/w—oil-in-water; CPPs—critical process parameters.