Figure 3.

Effects of NADH or NADPH on patulin transformation activity of G. oxydans strain 621 cell lysates. (A) Patulin (PAT) transformation activity (B) E-ascladiol formation. Reaction contains 500 µM NADH or NADPH, 10 µg/mL patulin, and 40 µL/mL cell lysate in 20 mM HEPES buffer, pH 6.5. Samples were taken every 3 h from a 0 h to 9 h time interval and were extracted with ethyl acetate. The organic phase was dried and then resuspended in 50 µL 10% acetonitrile solution before analysis by HPLC using a C18 column. Only lysates incubated with NADPH produced E-ascladiol. Data were from ● no lysate control, ■ lysate with NADPH, ▲ lysate with NADH and ▼ lysate, with no cofactors.