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HPLC chromatogram of assay mixture containing 500 μM NADPH and 10 μg/mL patulin in a 20 mM MES buffer, pH 5 with either (A) 10.3 μg GOX0525 (B) 47 μg GOX1899 or (C) no enzyme added. Reaction mixtures were incubated for 24 h at 30 °C before HPLC analysis. Patulin (1) and E-ascladiol (2) were eluted at 14.5 and 8-min retention times, respectively.
