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. 2022 Jul 15;23(14):7824. doi: 10.3390/ijms23147824

Figure 3.

Figure 3

Alteration of mitochondrial status in MIA-G cells treated with GEM. (A,B) Mitochondrial volume was assessed using MitoGreen staining. (C,D) Mitochondrial membrane potential was assessed using TMRE staining. (E,F) Mitochondrial ROS production was assessed using DHR123 staining. Scale bar: 50 μm. Fluorescence intensity was assessed by measuring the average value across 20 cells. Error bars represent standard deviations from three independent evaluations. * p < 0.05. GEM—gemcitabine; MIA-P—MIA-PaCa-2; MIA-G—MIA-PaCa-GEM; TMRE—tetramethylrhodamine ethyl ester; DHR—dihydrorhodamine; ROS—reactive oxygen species; NS, not significant.