TABLE 3.

Probability of detecting 10 spores of C. botulinum^a type B per sample^b using different selection methods and enrichment times prior to PCR

Enrichment time (h)	Detection probabilityc (%) with selection:
	Not performed	By heat treatment (70°C, 10 min)	By d-cycloserine (25 mg/ml)
0	0/12 (0)	0/12 (0)	0/12 (0)
18	2/12 (0.17)	5/12 (0.42)	2/12 (0.17)
24	5/12 (0.42)	3/12 (0.25)	2/12 (0.17)
30	0/12 (0)	0/12 (0)	3/12 (0.25)
36	2/12 (0.17)	6/12 (0.50)	2/12 (0.17)
48	2/12 (0.17)	5/12 (0.42)	3/12 (0.25)

^aStrains Eklund 2B and Eklund 17B. 

^bThe sample was a homogenate of 10 g of pig feces in 90 ml of TPGY broth (1:10 dilution). 

^cNo. of positive PCR results/no. of replicates tested (n = 12).