Figure 1.

Unknown cellular factors other than GILT inhibit amphotropic MLV-pseudotyped HIV-1-based vector infection in HeLa cells. (A) HeLa/control and HeLa/shGILT cells were treated with γ-IFN (0.2 μg/mL). Cell lysates were prepared 3 days after the treatment. The cell lysates were analyzed by Western blotting. (B) HeLa/control and HeLa/shGILT cells were treated with γ-IFN for 3 days and were inoculated with amphotropic MLV-pseudotyped HIV-1-based vector. Transduction titers in HeLa/control cells are always set to 1, and relative values ± SD are indicated. Asterisks indicate significant differences compared to PBS-treated cells (see Section 2). This experiment was performed in triplicate.