Table 3.
List of genes in the penzonemycin (pzm) biosynthetic gene cluster and their proposed functions. The DNA sequence of the pzm gene cluster has been deposited in GenBank (accession: ON345781).
| Gene | Size (aa) |
Annotation and Closest Homologue (Source, Accession No.) | % Identity/ Similarity |
|---|---|---|---|
| pzm1 | 353 | LysR family transcriptional regulator (Streptomyces halstedii LGO-A4, MBV7672918) | 94/94 |
| pzm2 | 83 | hypothetical protein (Streptomyces halstedii LGO-A4, MBV7672919) | 100/100 |
| pzm3 | 171 | N-acetyltransferase (Streptomyces halstedii LGO-A4, MBV7672920) | 98/98 |
| pzm4 | 298 | F420-dependent oxidoreductase (Streptomyces halstedii LGO-A4, MBV7672921) | 99/99 |
| pzm5 | 653 | AfsR/SARP family transcriptional regulator (Streptomyces sp. SM18, AWL43113) | 99/99 |
| pzm6 | 650 | anthranilate synthase (Streptomyces halstedii LGO-A4, MBV7672922) | 99/99 |
| pzm7 | 207 | isochorismatase (Streptomyces halstedii LGO-A4, MBV7672923) | 99/100 |
| pzm8 | 256 | 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (Streptomyces halstedii LGO-A4, MBV7672924) | 98/99 |
| pzm9 | 393 | 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthase(Streptomyces halstedii LGO-A4, MBV7672925) | 99/98 |
| pzm10 | 432 | transposase (Streptomyces halstedii LGO-A4, MBV7672926) | 99/99 |
| pzm11 | 459 | nitrosuccinate lyase, CreD homolog(Streptomyces cremeus NBRC 12760, BAU09301) | 67/73 |
| pzm12 | 659 | monooxygenase, CreE homolog (Streptomyces cremeus NBRC 12760, BAU09302) | 62/70 |
| pzm13 | 334 | quinone reductase (Streptomyces halstedii LGO-A4, MBV7672929) | 99/99 |
| pzm14 | 161 | Rrf2 family transcriptional regulator (Streptomyces halstedii LGO-A4, MBV7672930) | 100/100 |
| pzm15 | 406 | major facilitator superfamily (MFS) transporter (Streptomyces halstedii LGO-A4, MBV7672931) | 100/100 |
| pzm16 | 622 | acetolactate synthase (Streptomyces halstedii LGO-A4, MBV7672932) | 99/99 |
| pzm17 | 560 | Acyl-CoA ligase (Streptomyces halstedii LGO-A4, MBV7672933) | 99/99 |
| pzm18 | 527 | AMP-binding protein, CreM homolog (Streptomyces cremeus NBRC 12760, BAU09310) | 48/61 |
| pzm19 | 180 | SRPBCC family protein (Streptomyces halstedii LGO-A4, MBV7672935) | 99/99 |
| pzm20 | 1128 | discoidin domain-containing protein (Streptomyces halstedii LGO-A4, MBV7672936) | 99/99 |
| pzm21 | 126 | vicinal oxygen chelate (VOC) family protein (Streptomyces halstedii LGO-A4, MBV7672937) | 100/100 |
| pzm22 | 198 | RNA 2′,3′-cyclic phosphodiesterase (Streptomyces halstedii LGO-A4, MBV7672938) | 99/100 |