Figure 4.

Stimulation of MDA-MB-231 cells by TNFα + IL-1β and of BT-549 cells by IFNγ + IL-1β induces the activation of canonical transcription factors. (A) Activation of p65 was determined by kinetics analyses of TNFα and/or IL-1β stimulation in MDA-MB-231 cells (MDA) (cytokine concentrations as in Figure 1). (B) Activation of STAT1 (B1) and p65 (B2) was determined by kinetics analyses of IFNγ and/or IL-1β stimulation in BT-549 cells (BT) (cytokine concentrations as in Figure 2). Control cells were treated by the vehicle of the cytokines. STAT1 and p65 activation were determined by WB analysis. GAPDH was used as loading control. In each panel, a representative experiment of n = 3 is presented.