Figure 7.

Pro-inflammatory cytokines up-regulate the expression of cell-derived sTNFR2 by MDA-MB-231 cells, but have a divergent effect on cell-derived sTNFR1 expression levels. (A) MDA-MB-231 cells (MDA) were stimulated by TNFα and/or IL-1β (concentrations as in Figure 1) or treated by a vehicle control for 48 h. sTNFR1 (A1) and sTNFR2 (A2) levels were determined in CM of the cells by ELISA. In each panel, a representative experiment of n = 3 is presented. *** p < 0.001. ** p < 0.01. (B) MDA cells were stimulated by TNFα and/or IL-1β (concentrations as in Figure 1) or treated by a vehicle control for 5 h. TNFR1 mRNA (B1) and TNFR2 mRNA (B2) levels were determined by qPCR. In each panel, a representative experiment of n = 3 is presented. ** p < 0.01, * p < 0.05. NS, Not significant. Statistical analyses were performed as described in Section 2.