Skip to main content
. 2022 Jul 19;14(14):3513. doi: 10.3390/cancers14143513

Figure 8.

Figure 8

Pro-inflammatory cytokines up-regulate the expression of cell-derived sTNFR2 by BT-549 cells, but have a divergent effect on cell-derived sTNFR1 expression levels. (A) BT-549 cells (BT) were stimulated by IFN + IL-1β (concentrations as in Figure 2), by TNFα and/or IL-1β (concentrations as in Figure 1) or treated by a vehicle control for 48 h. sTNFR1 (A1) and sTNFR2 (A2) levels were determined in CM of the cells by ELISA. In each panel, a representative experiment of n = 3 is presented. *** p < 0.001, ** p < 0.01, * p < 0.05. NS, not significant. (B) BT cells were stimulated by IFNγ + IL-1β (concentrations as in Figure 2), TNFα and/or IL-1β (concentrations as in Figure 1) or treated by a vehicle control for 5 h. TNFR1 mRNA (B1) and TNFR2 mRNA (B2) levels were determined by qPCR. In Panel (B1), the data of TNFα and/or IL-1β stimulation demonstrate the average ± SD of n = 4 experiments, due to high variability between the assays. In all other figures of Panel (B), a representative experiment of n = 3 is presented. *** p < 0.001, ** p < 0.01, * p < 0.05. NS, not significant. Statistical analyses were performed as described in Section 2.