Table 1.

Experimental design.

Experiment	1	2	3		4
Factor	Host/Baits	Exposure Time	Mosquito Density		Replacement of Rabbit
Comparison	Human or membrane vs. rabbit with 100 mosquitoes	1 h vs. 12 h for human or membrane (within host)	50 vs 100 mosquitoes using rabbit		Rabbit with 100 mosquitoes vs. membrane with 50 mosquitoes
ITNs arms	Interceptor® G2 Unwashed Interceptor® G2 Washed 20× Interceptor® Unwashed Interceptor® Washed 20× Negative control		Interceptor® G2 Unwashed Interceptor® G2 Washed 20× Negative control	Interceptor® Unwashed Interceptor® Washed 20× Negative control	Interceptor® G2 Unwashed Interceptor® G2 Washed 20× Interceptor® Unwashed Interceptor® Washed 20× Negative control
Replicates per arm per comparison	5		15	15	15
Total replicates	75	100	90	90	150
Number of nights	15	10	10	10	16
Mosquitoes exposed	100		100, 50		100, 50
Host/bait	Rabbit, Human, Membrane	Human, Membrane	Rabbit		Rabbit-100, Membrane-50
Exposure time	12 h	12 h 1 h	12 h		12 h
Mosquito species	Anopheles arabiensis		Anopheles arabiensis	Anopheles gambiae	Anopheles arabiensis
Primary Outcomes	Blood feeding success (BFS), 24-h mortality (M24), 72-h mortality (M72)
Additional Outcome	Blood feeding Inhibition (BFI)