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Kinetic parameters. Different concentrations of oNPG were used as the substrate: (a) The kinetic parameters of BLGLB1; (b) BPGLB1; (c) E; different concentrations of lactose were used as the substrate: (d) BLGLB1; (e) E. The determination was carried out under the respective optimal reaction conditions. The concentration of BLGLB1, BPGLB1, and commercial β-galactosidase E was 0.87 μg/mL, 0.89 mg/mL and 0.04 mg/mL, respectively.
