Table 1.
The effect of metal ions on the activities of BLGLB1, BPGLB1, and E.
| Metallic Cations (5 mM) | BLGLB1 (%) | BPGLB1 (%) | E (%) |
|---|---|---|---|
| Untreated | 100 | 100 | 100 |
| Zn2+ | 103 ± 2.6 | 122 ± 2.5 | 147 ± 1.4 |
| Co2+ | 87 ± 4.2 | 186 ± 5.1 | 141 ± 1.0 |
| Al3+ | 44 ± 0.1 | 96 ±1.7 | 60 ± 3.9 |
| Ni2+ | 83 ± 3.6 | 212 ± 1.1 | 156 ± 3.7 |
| Fe2+ | 94 ± 0.5 | 104 ± 1.8 | 132 ± 5.6 |
| Mg2+ | 96 ± 2.5 | 154 ± 1.9 | 162 ± 6.2 |
| Na+ | 104 ± 0.9 | 167 ± 2.0 | 121 ± 3.2 |
| Ca2+ | 104 ± 0.3 | 111 ± 4.6 | 104 ± 9.7 |
| Mn2+ | 101 ± 0.6 | 101 ± 1.1 | 161 ± 2.0 |
| Li+ | 109 ± 1.1 | 129 ± 13.2 | 115 ± 6.3 |
The concentrations of BLGLB1, BPGLB1 and commercial β-galactosidase E are 0.87 μg/mL, 0.89 mg/mL, and 0.04 mg/mL respectively. The reactions are carried out under the respective optimal conditions.