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. 2022 Jul 13;11(14):2190. doi: 10.3390/cells11142190

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Co-cultivation of CAR T-cells with T47D tumour cells discordant for M-CSFR expression. (A) Flow cytometric analysis of cell surface M-CSFR expression in T47D and T47D FMS cells. (B) Engineered human T-cells were co-cultivated overnight at a 1:1 effector:target ratio with T47D (lacks M-CSFR) or T47D FMS (expresses M-CSFR) target cells. Tumour cell viability was evaluated using an MTT assay (mean ± SD, n = 3 technical replicates). Data are representative of 7 independent replicates, in which similar results were obtained. Supernatant collected at termination of these cultures was analysed for IFN-γ (C) and IL-2 (D) concentrations (mean ± SD, n = 3 biological replicates). Statistical analysis was undertaken using two-way ANOVA and selected differences are shown. **** p < 0.0001; NS–not significant.