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. 2022 Jul 15;23(14):7814. doi: 10.3390/ijms23147814

Figure 2.

Figure 2

Unkeito (UKT) affects tartaric acid-resistant phosphatase (TRAP) activity in a concentration-dependent manner and at early stage of osteoclast differentiation. (a) RAW 264 cells treated with sRANKL (100 ng/mL) with or without UKT (100 and 300 µg/mL) for five days (n = 4; scale bar = 25 µm); (b) RAW 264 cells (1 × 103 cells/96-well) treated with sRANKL (100 ng/mL) with or without UKT at 300 µg/mL for one, three, or five days (n = 4); (c) mRNA expression level of Nfatc1 normalized to that of Gapdh; (d) protein abundance of NFATc1 72 h after RANKL stimulation in the presence or absence of UKT (n = 3; scale bar = 20 µm); (e) intra-nuclear abundance of NFATc1. Error bars represent ± standard error of the mean. ** p < 0.01; *** p < 0.005; **** p < 0.0001, versus control groups on the same day. Black box: control; gray box: UKT; NC: negative control; ND: not detected.