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. 2022 Jul 15;23(14):7814. doi: 10.3390/ijms23147814

Figure 3.

Figure 3

Unkeito (UKT) suppresses nuclear factor of activated T cells 1 (NFATc1) through the Blimp1–Bcl6 axis by increasing Bcl6 expression. (a) mRNA expression level of Bcl6 normalized to that of Gapdh; (b) abundance of Bcl6 72 h after RANKL stimulation in the presence or absence of UKT (n = 3; scale bar = 10 µm, 2 µm); (c) relative fluorescence intensity of Bcl6; (d) mRNA expression level of Blimp1 normalized to that of Gapdh; (e) protein level of Blimp1 72 h after RANKL stimulation in the presence or absence of UKT (n = 3; scale bar = 10 µm, 2 µm); (f) intra-nuclear level of Blimp1. Error bars represent ± standard error of the mean. ** p < 0.01, *** p < 0.005, versus control groups on the same day. Black box: control; gray box: UKT; NC: negative control; ND: not detected.