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Unkeito (UKT) reduces p65 expression in the NF-κB signaling pathway. Expression of (a) p65 and (b) p50 normalized to that of Gapdh; (c) p65 localization 72 h after RANKL stimulation in the presence or absence of UKT (n = 3; scale bar = 10 µm, 2 µm); (d) p65 abundance in the nucleus. Error bars represent ± standard error of the mean. * p < 0.05; ** p < 0.01, versus control groups on the same day. Black box: control; gray box: UKT; NC: negative control; ND: not detected.
