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. 2022 Jul 16;14(14):3459. doi: 10.3390/cancers14143459

Figure 3.

Figure 3

Thermo-sensitive Pluronic F-127 hydro-gel#30 possesses better carrier characteristics for “no drip” topical applications of AKRO-QC-ICG imaging probe under force of gravity. Analysis of behavior of carriers of the AKRO-QC-ICG imaging probe. Both AKRO-QC-ICG (final 5 μM) dissolved in 50 μL of Pluronic F-127 hydro-gel#30 and a paper applicator impregnated with AKRO-QC-ICG (final 5 μM in 50 μL of DMSO) were topically applied to a plastic surface that was pre-heated at 37 °C. (A)—Distribution of the AKRO-QC-ICG in the carriers on the flat surface. (B)—Distribution of the AKRO-QC-ICG in the carriers on the surface with a 30 degree slope after 1 min. Scale bar 5 mm. (C)—Analysis of the levels of auto-fluorescence of AKRO-QC-ICG at the upper and bottom quadrant of the applied carrier in the regions of interest (ROIs). Data were normalized to background. Notes: †—p < 0.05 compared to ROI-3 in (B) with slope (Student’s t-test in three independent experiments). Imaging and analysis—Lab-FLARE® imaging system with 800 nm filter set and Resvet Imaging software (both Curadel, LLC, Natick, MA, USA). It should be noted that the lower concentration of probe used here is quenched by gel formulation, but not when formulated in 100% DMSO. Quantitation of signal reveals lack of probe movement when formulated using gel#30.