Table 1.
Mass spectrometric identification of dengue virus proteins in the supernatant of BHK-21 cells transfected with different DNA vaccines.
| Gel Fraction (a) | DNA Vaccine (b) | Protein (c) | Mass (d) | UniquePep (e) | UniqueSC (f) | NSAF (g) | %Coverage (h) | PtnScore (i) |
|---|---|---|---|---|---|---|---|---|
| F3-F4 | pcTPANS1 | NS1 | 42,525 | 87 | 627 | 0.2637 | 84 | 206.65 |
| F7-F8 | pE1D2 | E | 46,881 | 31 | 85 | 0.0379 | 67 | 82.73 |
| F9-F10 | pE1D2 + pcTPANS1 | NS1 | 42,525 | 66 | 484 | 0.1838 | 80 | 168.49 |
| E | 46,881 | 31 | 71 | 0.0239 | 65 | 80.26 | ||
| F11-F12 | pNS1/E/D2 | NS1 | 42,525 | 25 | 58 | 0.0293 | 55 | 53.17 |
| E | 46,881 | 30 | 62 | 0.0278 | 61 | 80.38 |
(a) SDS-PAGE fraction used for in-gel digestion and protein identification by nLC-MS/MS; (b) plasmid DNA used to transfect BHK-21 cells; (c) name of the identified protein; (d) theoretical molecular mass of the protein in kDa; (e) number of identified peptides exclusive to the protein sequence; (f) total number of spectral counts of all identified unique peptides mapping to the protein sequence; (g) relative protein abundance based on the “Normalized Spectral Abundance Factor”; (h) percentage of identified sequence residues of the protein; and (i) protein confidence identification score calculated by the algorithm PatternLab V.