Figure 9.

Effects of genistein on NF-κB nuclear translocation in (A,B) HT29 cells and (C,D) SW620 cells determined by immunocytochemistry. The measurements were made with 0.1% DMSO (control cells, (A,C)) or genistein 100 μM (B,D) treatment for 48 h. The fluorescence was monitored with a Leica TCS-SPE Confocal Microscope, using 63× immersion oil (147 N.A.) objective lens. Scale bar 10 μm. White square in the bottom left corner shows the whole field without zoom and the image in the center shows a zoom from the white square image. Nucleus and NF-κB proteins are represented in the picture in blue and red, respectively. The merge between both (pink) indicates that NF-κB had translocated into the nucleus.