Table 1.
Primers sequences and their respective annealing temperatures and accession number.
| Gene | Forward Primer (5′-3′) Reverse Primer (5′-3′) |
Annealing Temperature (°C) | Accession Number |
|---|---|---|---|
| B2M | 5′-TTT CAT CCA TCC gAC ATT GA-3′ 5′-Cgg CAg gCA TAC TCA TCT TT-3′ |
54 | NM_004048 |
| SOD2 | 5′-CgT gCT CCC ACA CAT CAA TC-3′ 5′-TgA ACg TCA CCg Agg AgA Ag-3′ |
64 | BT006967 |
| SOD1 | 5′-TCA ggA gAC CAT TgC ATC ATT-3′ 5′-CgC TTT CCT gTC TTT gTA CTT TCT TC-3′ |
64 | NM_000454 |
| CAT | 5′-CAT CgC CAC ATg AAT ggA TA-3′ 5′-CCA ACT ggg ATg AgA ggg TA-3′ |
61 | NM_001752 |
| GPX1 | 5′-gCg gCg gCC Cag TCg gTg TA-3′ 5′-gAg CTT ggg gTC ggT CAT AA-3′ |
61 | M21304 |
| PPARGC1A | 5′-TCA gTC CTC ACT ggT ggA CA-3′ 5′-TgC TTC gTC gTC AAA AAC Ag-3′ |
60 | AF106698 |
| ESRRA | 5′-TCg CTC CTC CTC TCA TCA TT-3′ 5′-Tgg CCA AAC CCA AAA ATA AA-3′ |
52 | NM_004451 |
| TFAM | 5′-gTg gTT TTC ATC TgT CTT ggC-3′ 5′-ACT CCg CCC TAT AAg CAT CTT-3′ |
60 | BT019658 |
| SSBP1 | 5′-TgT gAA AAA ggg gTC TCg AA-3′ 5′-Tgg CCA AAg AAg AAT CAT CC-3′ |
60 | AF277319 |
| TNF | 5′-AAg CCT gTA gCC CAT gTT gT-3′ 5′-ggA CCT ggg AgT AgA TgA ggT-3′ |
58 | NM_000594 |
| IL1B | 5′-TCg CCA gTg AAA TgA Tgg CT-3′ 5′-ggT Cgg AgA TTC gTA gCT gg-3′ |
58 | BT007213 |
| CXCL8 | 5′-ggC ACA AAC TTT CAg AgA CAg CAg-3′ 5′-gTT TCT TCC Tgg CTC TTg TCC TAg-3′ |
66 | AK311874 |
| CXCR2 | 5′-AgT TCT Tgg CAC gTC ATC gT-3′ 5′-CCC CTg AAg ACA CCA gTT CC-3′ |
57 | M68932 |
| HPSE | 5′-gCA AAC TgC TCA ggA CTg gA-3′ 5′-gCT gAC CAA CAT CAg gAC CA-3′ |
60 | AF084467 |
| IL10 | 5′-ACA TCA Agg CgC ATg TgA AC-3′ 5′-CAC ggC CTT gCT CTT gTT TTC-3′ |
60 | M57627 |
| PPARG | 5′-gAg CCC AAg TTT gAg TTT gC-3′ 5′-CTg TgA ggA CTC Agg gTg gT-3′ |
61 | BT007281 |
| mtDNA | 5′-CgT gAC TCC TAC CCC TCA CA-3′ 5′-ATC ggg TgA TAg CCA Ag-3′ |
60 | NM_025230.5 |
| 18S | 5′-ggA CAC ggA CAg gAT TgA CA-3′ 5′-ACC CAC ggA ATC gAg AAAGA |
60 | NR_146119.1 |