FIGURE 2.

(A) SynthoPlate (SP) mimics platelet adhesion and aggregation mechanisms by binding to vWF vis vWF‐binding peptide (VBP), to collagen via collagen‐binding peptide (CBP), and to active platelet GPIIb‐IIIa via fibrinogen‐mimetic peptide (FMP); (B) Microfluidic set‐up and representative fluorescence microscopy images, where calcein‐stained (green) platelets and soluble vWF in plasma is flowed over collagen‐coated channel at high shear (60 dyn cm⁻²): PRP was able to form large platelet aggregates on the channel surface, and this was significantly reduced with PPP; introducing Rhodamine B labeled (red) SP in PPP significantly rescued platelet aggregate formation (red SP colocalization with green platelets shown in yellow); (C) shows representative lumi‐aggregometry results of SP effect on platelet aggregation where Platelet (Plt): SP ratio of 1:10 to 1:100 increased platelet aggregation, below this ratio (Plt: SP = 1:1) had negligible effect, while above this ratio (Plt: SP = 1:1000) reduced aggregation possibly due to dilution effect; (D) Representative results in mouse tail‐clip bleeding model shows that induction of thrombocytopenia (TCP) in mice significantly increased bleeding time, and treatment of this TCP condition with SP reduced bleeding time significantly