Figure 1.
Illustrative examples of C(sp 3 )−H and C(sp 2 )−H hydroxylations catalysed by cytochrome P450s. A) regioselective allylic hydroxylation of cylcohexene‐1‐carboxylic acid methyl ester. B) CYP101B1‐catalysed hydroxylation of cyclooctyl acetate. C) P450BM3 mutant A74G/L188G catalyses allylic hydroxylation of ethyl 6‐heptenoate. D) Hydroxylations of halogenated benzenes, toluene and anisole using P450BM3 mutant M2. E) P450BM3 mutants catalyse hydroxylation of anilides to mostly 4‐hydroxy derivatives. F) Hydroxylation of benzene to hydroquinone using P450BM3 double mutant A82F/A328F.