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. 2022 May 2;17(12):e202200115. doi: 10.1002/cmdc.202200115

Figure 9.

Figure 9

Applications of oxygenating biocatalysts in biocatalytic cascades for drug discovery and development. A) Enzymatic one‐pot synthesis of cycloalkanones using P450BM3‐ADH cascade. Oxidised cofactor NADP+ is used in ADH‐catalysed oxidation of propanol as sacrificial substrate to generate NADPH in situ. B) P450BM3‐ADH cascade employed for selective allylic oxidation of (+)‐valence (67) to high‐value (+)‐nootkatone (66), an additive used in pharmaceuticals. Simultaneous butanol oxidation catalysed by ADH used to generate NADH. C) Three‐enzyme cascade used for the amination of unfunctionalised cycloalkanes. D) Fe/αKG SadA used in cascade with LasA for the synthesis L‐threo‐βOH‐Leu (70), a precursor for cyclic depsipeptide.