Fig. 4.
External application of abscisic acid (ABA) to soybean (Glycine max) flowers results in stomatal closure, and sealing of stomata results in elevated flower temperature under conditions of water deficit (WD) and heat stress (HS) combination. (a) Stomatal aperture of sepals from plants subjected to control (CT), HS, WD or WD + HS conditions at 60 min following application of 0 or 50 μM ABA. (b) Inner flower temperature of flowers from plants subjected to CT, WD, HS or WD + HS, coated or uncoated with a thin layer of petroleum jelly (PTJ) for 120 min. All experiments were conducted with three biological repeats, each with 10 plants as technical repeats. Results are shown as box‐and‐whisker plots with borders corresponding to the 25th and 75th percentiles of the data. Different letters denote significance at P < 0.05 (ANOVA followed by a Tukey's post hoc test).