Fig. 6.
Enhanced expression of transcripts encoding the abscisic acid (ABA) degradation enzyme ABA 8′‐hydroxylase in flowers from soybean (Glycine max) plants subjected to heat stress (HS) or water deficit (WD) combined with HS, and higher resistance of these flowers to external ABA application. (a) Heat maps and a pathway showing the expression of transcripts involved in ABA biosynthesis and degradation in whole flowers from plants grown under control (CT), WD, HS or WD + HS conditions. All transcripts shown are significant at P < 0.05 (negative binomial Wald test followed by Benjamini–Hochberg correction). (b) Stomatal aperture of sepals from plants subjected to CT, HS, WD or WD + HS at 60 min following application of 30, 15, 7.5 or 0 µM ABA. All experiments were conducted with three biological repeats, each with 10 plants as technical repeats. Twenty microscopic fields from all parts of sepals were measured for each plant. Results are shown as box‐and‐whisker plots with borders corresponding to the 25th and 75th percentiles of the data. Different letters denote significance at P < 0.05 (ANOVA followed by a Tukey's post hoc test).