Fig. 8.
The effect of water deficit (WD) and heat stress (HS) combination on flower and leaf stomatal aperture, transpiration and temperature in tobacco (Nicotiana tabacum). (a) Leaf temperature of tobacco plants subjected to control (CT), HS, WD or WD + HS. (b) Stomatal aperture of tobacco leaves subjected to CT, WD, HS or WD + HS. (c) Transpiration (E) of tobacco leaves subjected to CT, WD, HS or WD + HS. (d–f) As (a–c), but for tobacco flowers from plants subjected to CT, WD, HS or WD + HS. All experiments were conducted with three biological repeats, each with 10 plants as technical repeats. Twenty microscopic fields from all parts of sepals or from the middle section of leaves (between the main veins) were measured for each plant. Results are shown as box‐and‐whisker plots with borders corresponding to the 25th and 75th percentiles of the data. Different letters denote significance at P < 0.05 (ANOVA followed by a Tukey's post hoc test).