FIG. 5.

Relative yields of wild-type and dca mutant Acinetobacter strains grown at the expense of dicarboxylic acids of different chain length. Strains represented in the legend are ADP1, wild type; ADP8018, ΔdcaH1; ADP8062, ΔdcaE2; and ADP8061, ΔdcaA2. Dicarboxylic acids of different chain length are C₆, adipic acid; C₇, pimelic acid; C₈, suberic acid; C₁₀, sebacic acid; C₁₃, tridecanedioic acid; and C₁₄, tetradecanedioic acid. Adipic acid was supplied at 2 mM, and other dicarboxylic acids were supplied at a concentration that provided an equivalent molarity of carbon atoms. The optical density of a mutant culture on each substrate was measured at the time that wild-type cells, grown in parallel on the same substrate, reached a plateau in density. For two of the dicarboxylic acids (chain length C₇ and C₁₄), this required an extra overnight period of growth.