TABLE 2.
Growth phenotypes of E. coli strains with respect to 4-hydroxybenzoate
| E. coli strain and plasmid | Growth at the expense of 4-hydroxybenzoatea |
|---|---|
| DH5α | − |
| DH5α(pZR8000) | − |
| DH5α(pZR8000′)b | − |
| EDP1 | − |
| EDP1(pZR8000) | + |
| EDP1(pZR8000′) | + |
a
Cells were grown in minimal medium supplemented with 0.1% yeast extract and chloramphenicol to maintain plasmids. Tubes with 5 mM sodium 4-hydroxybenzoate were compared to those containing no additional carbon source as well as those with 5 mM sodium chloride.
b
pZR8000′ is the plasmid isolated from mutant EDP1(pZR8000), which grows at the expense of 4-hydroxybenzoate.