TABLE 4.
Dicarboxylic acid pathway genes and their homologs in flanking regions of the ADP1 chromosome
| dca gene of homolog(s) | % G+C
content
|
% nta identity | Distance (kb) between dca gene and homolog | Function(s) of gene product | % aab identity (% similarity) | GenBank accession no. or reference | |
|---|---|---|---|---|---|---|---|
| dca gene | Homolog | ||||||
| dcaI | 40 | Putative Dcac succinyl-CoA transferase, α chain | This study | ||||
| pcaI | 55 | 61 | 8.4 | β-Ketoadipate succinyl-CoA transferase, α chain | 61 (75) | AAC46432 | |
| dcaJ | 41 | Putative Dca adipate succinyl-CoA transferase, β chain | This study | ||||
| pcaJ | 54 | 65 | 8.4 | β-Ketoadipate succinyl-CoA transferase, β chain | 68 (80) | AAC37147 | |
| dcaF | 44 | Putative Dca-CoA thiolase | This study | ||||
| pcaF | 56 | 59 | 16 | β-Ketoadipyl-CoA thiolase | 56 (70) | AAC37148 | |
| dcaK | 41 | Putative Dca transport | This study | ||||
| mucK | 40 | 63 | 12d | cis,cis-Muconate transport; possible adipate transport | 55 (72) | AAC27117 | |
| pcaK | 42.5 | Divergent | 13.5 | Protocatechuate transport | 21 (37) | AAC37151 | |
a
nt, nucleotide.
b
aa, amino acid.
c
Dca, dicarboxylic acid.
d
Based on sequence analysis of DNA between mucK and dcaF (D. Parke, unpublished data).