Figure 2.

Evaluation of antibody titers, avidity and isotype by ELISA. Sera of Balb/c mice after 3-dose DNA immunization were assayed for Pfs25-specific and PfCSP-specific antibodies using recombinant proteins of Pfs25 and PfCSP. Antibody titers are reported as the geometric mean (GM) with 95% confidence intervals (CI). (A,B) PfCSP-specific antibody responses in female (A) and male (B) Balb/c mice. (C,D) Pfs25-specific antibody responses in female (C) and male (D) Balb/c mice. Statistical analyses were performed using the Mann–Whitney test, and p values are indicated. (E) Antibody avidity was assayed in the pooled mouse sera of each group, and the numbers indicate NaSCN molar concentration required for 50% dissociation of bound antibodies. (F) Antibody isotypes were tested in pooled mouse sera of each group and compared as a ratio of IgG1 to IgG2a.