Table 1.

Primers and probes used for real-time PCR assays targeting tick taxa, Francisella, and Rickettsia.

Organism	Genus/Group/ Species	Target Gene	PCR ID	Name	Sequence (5′ → 3′)	Amplicon (bp)	Reference
Tick		12S rDNA	12S	T1B	AAA CTA GGA TTA GAT ACC CT	320	[43]
				T2A	AAT GAG AGC GAC GGG CGA TGT
Francisella		fopA		Forward	GGC AAA TCT AGC AGG TCA AGC		[40]
	Francisella		FopA	Reverse	CAA CAC TTG CTT GAA CAT TTC TAG	89
				Probe	GGT GCT TGG GAT GTG GGT GGT G
		sucC		Forward	AAC TGG CTG ACC TTC AGC AT		[41]
	Francisella		GF1	Reverse	GTG GTC GTG GTA AAG CTG GT	125
				Probe	CCG ATT AGG CTT TCT GCT ACT TCA CGA
		lpnA	Tul4	Forward	ACC CAC AAG GAA GTG TAA GAT TA	76	[40]
	F. tularensis			Reverse	GTA ATT GGG AAG CTT GTA TCA TG
				Probe	AAT GGC AGG CTC CAG AAG GTT CTA AGT
				Forward	CGC AGG TTT AGC GAG CTG TT
	F. tularensis	lpnA	iQFt1	Reverse	GCA GCT TGC TCA GTA GTA GCT GTC T	108	[42]
				Probe	CAT CAT CAG AGC CAC CTA ACC CTA
Rickettsia	SFG	gltA	GltA	SFG_gltA_F	CCT TTT GTA GCT CTT CTC ATC C	145	[40]
				SFG_gltA_R	GCG ATG GTA GGT ATC TTA GCA A
				SFG_gltA_P	TGG CTA TTA TGC TTG CGG CTG TCG GT
	Rickettsia	17 kDa	17 kDa	Rr17 kDa.61p	GCT CTT GCA AC TTC TAT GTT	434	[45]
				Rr17 kDa.492n	CAT TGT TCG TCA GGT TGG CG

bp—base pairs; 12S rDNA—12S ribosomal DNA gene; fopA—gene encoding the outer membrane protein A (FopA); sucC—gene encoding the succinyl-CoA ligase [ADP-forming] subunit beta; lpnA—gene encoding the lipoprotein A (LpnA); gltA—gene encoding citrate synthase; SFG—spotted fever group; 17 kDa—17 kilo Dalton surface antigen.