Skip to main content
. 2022 Jul 11;12(7):633. doi: 10.3390/metabo12070633

Figure 2.

Figure 2

Mobile phase optimization for isobaric compounds chromatography separation. The GUDCA, GHDCA, GCDCA, and GDCA have the same molecular formula, C26H43NO5 ([M − H]− m/z 448.3063), but different -OH positions. (a) 2 mM ammonium acetate in aqueous solvent; (b) 2mM ammonium acetate and 0.05% formic acid in aqueous solvent; (c) 2 mM ammonium acetate and 0.01% formic acid in aqueous solvent.