Figure 8.

Assessing LOH frequency of two distinct C. albicans chromosomes within a single strain. (A) Illustration of the strain possessing the BFP/GFP LOH reporter system on Chr5L and the GAL1/URA3 system of Chr1L. Centromeres are indicated by ovals and distances between reporter systems and centromeres are indicated in gray. (B) Median frequencies of mono-fluorescence, LOH frequency, in YPD (black), and 10 µg/mL fluconazole (green), +/− standard deviation. LOH frequencies were assessed by fluctuation analysis (two independent strains, n = 6). Fold changes of median LOH frequencies, between YPD and fluconazole conditions are indicated on the plot in gray and statistical differences were evaluated using t-test (NS p > 0.05 and **** p < 0.0001).