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. Author manuscript; available in PMC: 2022 Dec 14.
Published in final edited form as: Immunity. 2021 Nov 16;54(12):2772–2783.e5. doi: 10.1016/j.immuni.2021.10.005

Figure 4. Kynurenine upregulates CD28 in LLPC.

Figure 4.

A) Representative histogram of AhR protein expression in PC from the BM of a WT mouse compared to fluorescence minus one (FMO), representative of 4 individual experiments. B) Fold change of Cyp1a1, Mcl-1, Atf4, and Cd28 mRNA expression from purified BM PC cultured in media with or without 100 μM kynurenine, (mean ± SD) n = 3, statistical analysis by Student’s t test. This data is representative of 3 individual experiments. C) MFI of CD28 on purified BM PC from WT and CD28-AYAA KI mice in co-culture with BMDC with or without kyn (100 μM) (mean ± SD), statistical analysis by Student’s t test. Data is aggregated from 2 individual experiments. *p<0.05, **p<0.01, ***p<0.001, ns = not significant. See also sFig. 6.