KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| AhR Monoclonal Antibody (4MEJJ)- PE | eBioscience/ThermoFisher Scientific | 12-5925-82 |
| IDO Monoclonal Antibody (mIDO-48), eFluor 660 | eBioscience/ThermoFisher Scientific | 50-9473-82 |
| Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor 660 | eBioscience/ThermoFisher Scientific | 50-4031-82 |
| APC anti-mouse CD3 | Biolegend | 100236 |
| FITC anti-mouse CD3 | Biolegend | 100203 |
| APC anti-mouse CD43 | Biolegend | 143207 |
| APC/Cyanine 7 anti-mouse/human CD45R/B220 | Biolegend | 103223 |
| Brilliant Violet 421 anti-mouse/human CD45R/B220 | Biolegend | 103240 |
| Brilliant Violet 421 anti-mouse CD24 | Biolegend | 101825 |
| FITC anti-mouse IgM | Biolegend | 406505 |
| PE anti-mouse IgD | Biolegend | 405705 |
| Brilliant Violet 605 anti-mouse CD184 (CXCR4) | Biolegend | 146519 |
| PerCP/Cyanine 5.5 anti-mouse CD93 | Biolegend | 136511 |
| FITC anti-mouse CD19 | Biolegend | 152403 |
| Pacific Blue anti-mouse/human CD11b | Biolegend | 101223 |
| APC/Cynanine 7 anti-mouse CD3 | Biolegend | 100221 |
| PerCP/Cyanine 5.5 anti-mouse CD45R/B220 | Biolegend | 103235 |
| APC anti-mouse CD19 | Biolegend | 152409 |
| PE anti-mouse CD21/CD35 (CR2/CR1) | Biolegend | 123409 |
| PerCP/Cyanine 5.5 anti-mouse CD4 | Biolegend | 100539 |
| APC/Cyanine 7 anti-mouse CD8b.2 | Biolegend | 140421 |
| PE anti-mouse CD4 | Biolegend | 116005 |
| PE anti-mouse CD28 | Biolegend | 102106 |
| PerCP/Cyanine 5.5 anti-mouse CD28 | Biolegend | 102114 |
| APC anti-mouse CD138 (Syndecan-1) | Biolegend | 142506 |
| PE anti-mouse CD138 (Syndecan-1) | Biolegend | 142504 |
| PE anti-mouse F4/80 | Biolegend | 123109 |
| PE anti-mouse CD11c | Biolegend | 117307 |
| Brilliant Violet 785 anti-mouse CD11c | Biolegend | 117335 |
| PE anti-mouse CD80 | Biolegend | 104708 |
| PE anti-mouse CD86 | Biolegend | 105007 |
| PE anti-mouse/human CD11b | Biolegend | 101207 |
| PE anti-mouse I-A/I-E (MHC class II) | Biolegend | 107607 |
| PE anti-mouse CD40 | Biolegend | 124609 |
| APC/Cyanine 7 Goat anti-mouse IgG (minimal x-reactivity) | Biolegend | 405316 |
| APC anti-mouse CD45 | Biolegend | 103111 |
| FITC anti-mouse CD45 | Biolegend | 103107 |
| Pacific Blue anti-mouse CD45 | Biolegend | 103125 |
| APC/Cyanine 7 anti-mouse CD45 | Biolegend | 103115 |
| PE anti-mouse CD45 | Biolegend | 103106 |
| Brilliant Violet 421 anti-mouse CD45 | Biolegend | 103134 |
| Brilliant Violet 785 anti-mouse CD45 | Biolegend | 103149 |
| PerCP/Cyanine 5.5 anti-mouse CD45 | Biolegend | 103131 |
| Live/Dead Fixable Blue Dead Cell Stain Kit, for UV excitation | ThermoFisher Scientific | L23105 |
| Live/Dead Fixable Near-IR Dead Cell Stain Kit | ThermoFisher Scientific | L10119 |
| Cell Trace Violet Cell Proliferation Kit | ThermoFisher Scientific | C34571 |
| CD138+ Plasma Cell Isolation Kit, mouse | Miltenyi Biotec | 130-092-530 |
| Mojosort Mouse CD11c Nanobeads | Biolegend | 480077 |
| Bacterial and virus strains | ||
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| NP-PE (phycoerythrin) | Biosearch Technologies | N-5070-1 |
| NP-KLH (Keyhole Limpet Hemocyanin) | Biosearch Technologies | N-5060-5 |
| NP-OVAL (Ovalbumin) | Biosearch Technologies | N-5051-100 |
| Blocker Casein in PBS | ThermoFisher Scientific | 37528 |
| eBioscience Intracellular Fixation & Permeabilization Buffer Set | eBioscience/ThermoFisher Scientific | 88-8824-00 |
| 4-(Dimethylamino)benzaldehyde | Millipore Sigma | 156477 |
| Substrate Reagent Pack | R&D systems | DY999 |
| Diphtheria Toxin from Corynebacterium diphtheriae | Millipore Sigma | D0564 |
| Imject Freund’s Complete Adjuvant (FCA) | ThermoFisher Scientific | 77140 |
| Imject Freund’s Incomplete Adjuvant (FIA) | ThermoFisher Scientific | 77145 |
| Imject Alum Adjuvant | ThermoFisher Scientific | 77161 |
| Epacadostat | Chemietek | CT-EPAC |
| Recombinant Mouse IFN-γ research grade | Miltenyi Biotech | 130-105-790 |
| Recombinant mouse GM-CSF research grade | Miltenyi Biotech | 130-094-043 |
| L-Kynurenine | Millipore Sigma | 2922-83-0 |
| L-Tryptophan | Millipor Sigma | 73-22-3 |
| PowerUp SYBR Green Master Mix | ThermoFisher Scientific/Applied Biosystems | A25779 |
| Critical commercial assays | ||
| Mouse IgG ELISA Quantitation Kit | Bethyl Laboratories Inc. | E90-131 |
| Mouse IgG ELISpot Basic Kit (HRP) | Mabtech | 3825-2H |
| RNeasy Mini Kit | Qiagen | 74106 |
| iScript cDNA Synthesis Kit | BioRad | 1708890 |
| Deposited data | ||
| Experimental models: Cell lines | ||
| J558 | ATCC | TIB-6 |
| Experimental models: Organisms/strains | ||
| C57BL/6J | Jackson Labs | 000664 |
| C57BL/6NCrl | Charles River Labs | 027 |
| B6.129-Ido1tm1Alm/J | Jackson Labs | 005867 |
| B6.FVB-1700016L21 RikTg(Itgax0DTR/EGFP)57Lan/J | Jackson Labs | 004509 |
| B6.129S2-Cd28tm1Mak/J | Jackson Labs | 002666 |
| B6.129X1-Cd28tm1Jmg/Mmjax | Jackson Labs | 32039-JAX |
| B6.129S4-Cd80tm1ShrCd86tm2Shr/J | Jackson Labs | 003610 |
| C57BL/6-Tg(UBC-GFP)30Scha/J | Jackson Labs | 004353 |
| Oligonucleotides | ||
| Murine GAPDH: FWD: ‘CTT TGT CAA GCT CAT TTC CTG G’ REV: ‘TCT TGC TCA GTG TCC TTG C’ |
ThermoFisher Scientific | Custom |
| Murine Cyp1a1: FWD: ‘AGA GCA CTA CAG GAC ATT TGA G’ REV: ‘CCA AAG AGG TCC AAA ACA ATC G’ |
ThermoFisher Scientific | Custom |
| Murine Cd28: FWD: ‘GCC TTA CCT AGA CAA CGA GAG’ REV: ‘CAA GCC ATA ACA AAA CAG GAC TC’ |
ThermoFisher Scientific | Custom |
| Murine Mcl-1: FWD: ‘TTG TAA GGA CGA AAC GGG AC’ REV: ‘TCT AGG TCC TGT ACG TGG AAG’ |
ThermoFisher Scientific | Custom |
| Murine Atf-4: FWD: ‘GGT TCT CCA GCG ACA AGG’ REV: ‘GCA TCG AAG TCA AAC TCT TTC AG’ |
ThermoFisher Scientific | Custom |
| Murine Blimp-1: FWD: ‘ATT AAG CCT ATC CCT GCC AAC’ REV: ‘CTA CTG TAT TGC TTT GGG TTG C’ |
ThermoFisher Scientific | Custom |
| Murine DTR-GFP: FWD: ‘AGT GCT TCA GCC GCT ACC’ REV: ‘GAA GAT GGT GCG CTC CTG’ |
ThermoFisher Scientific | Custom |
| Recombinant DNA | ||
| Software and algorithms | ||
| Prism Graph Pad version 6–8 | Prism | https://www.graphpad.com/scientific-software/prism/ |
| Flow Jo | BD Biosciences | https://www.flowjo.com/solutions/flowjo |
| Other | ||