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. 2022 Jul 13;23(14):7743. doi: 10.3390/ijms23147743

Figure 1.

Figure 1

dsVDUP1-834 transfection induces VDUP1 expression in a dose-dependent manner in A549 cells. (A) Schematic representation of VDUP1 promoter showing target site and sequences of saRNAs. A549 cells were transfected with dsCon or indicated concentrations of saRNAs for 96 h. (B) The mRNA expression of VDUP1 was determined by qRT-PCR. (C) The protein expression of VDUP1 was assessed by Western immunoblot analysis. Normalized relative intensities are shown below the bands. (D) OAS1 and OAS3 mRNA expression was analyzed by qRT-PCR. Data are represented as mean ± S.D of triplicate determinations. * denotes that the response is significantly different from mock-treated group as determined by Dunnett’s t-test at p < 0.05.