Figure 2.

dsVDUP1-834 transfection inhibits cell growth and induces cell-cycle arrest in A549 cells. A549 cells were transfected with dsCon or indicated concentrations of saRNAs. (A) After 96 h, cell proliferation was measured by XTT assay. Data are represented as mean ± S.D of triplicate determinations. (B) Cell-cycle distribution was analyzed using flow cytometry 72 h after transfection. (C) A representative histogram of cell-cycle analysis. Black triangles indicate the peaks of each histogram. (D) Protein levels of p27, p53, cyclin A, cyclin B1, and β-actin in total cell lysates were determined by Western immunoblot analysis. Normalized relative intensities are shown below the bands. * denotes that the response is significantly different from mock-treated group as determined by Dunnett’s t-test at p < 0.05.