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. 2022 Jul 13;23(14):7743. doi: 10.3390/ijms23147743

Figure 5.

Figure 5

The effects of dsVDUP1-834 were attenuated by Ago2 siRNA in A549 cells. A549 cells were transfected with dsCon, dsVDUP1-834 (10 nM), or siAgo2 (10 nM) for 96 h. (A) ChIP assays were performed using control IgG or anti-Ago2 antibody to pull down associated DNA. The precipitated DNA was amplified by PCR using primer sets specific to dsVDUP1-834 target sites. (B) The mRNA expression of VDUP1 was analyzed by qRT-PCR. Data are represented as mean ± S.D of triplicate determinations. (C) Cell proliferation was measured by XTT assay. Data are represented as mean ± S.D of triplicate determinations. (D) Protein levels of p27, p53, and β-actin in total cell lysates were determined by Western immunoblot analysis. Normalized relative intensities are shown below the bands. * denotes that the response is significantly different from dsVDUP1-834-treated group as determined by Dunnett’s t-test at p < 0.05.