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. 2022 Jul 8;11(14):3974. doi: 10.3390/jcm11143974

Figure 2.

Figure 2

The expression and cellular distribution of PDKs in rat brains after SAH. Representative immunofluorescence staining of PDK4, Iba1 (a microglial marker) (A), NeuN (a neuronal marker) (B), and GFAP (an astrocytic marker) (C) in the cortex of the right temporal lobe after SAH (PDK4, green; NeuN, Iba1, and GFAP, red; and DAPI, blue). (D) Representative bands of PDK1, PDK2, PDK3, PDK4, PDH, and p-PDH expression in the cortex at each time point (0, 6, and 24 h and 2, 3, and 7 d) after SAH. (E) Quantitative analysis of Western blot results showed that the ratio of p-PDH/PDH was significantly increased after SAH. (F,G) Quantitative analysis of Western blot and qPCR results showed that variation of PDKs protein and mRNA levels after SAH. Bars represent the means ± SD. * p < 0.05; ** p < 0.01; vs. sham (n = 5 in each group). Bar = 50 μm.