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Analytical features of methylated tramadol. (A) Extracted ion chromatogram of urine extract at m/z 278.2120 corresponding to [M + H]+ ion of methyl tramadol. MS/MS spectra of [M + H]+ ion of (B) N-methyl tramadol and (C) O-methyl tramadol. The product ion at m/z 72.0816 correspond to a dimethylaziridium ion and allow for the annotation of N-methyl tramadol.
