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Molecular network generated under negative ion mode. (A) Global overview and (B) focus on the C2 cluster of glucurono-conjugated molecules including tramadol. MS/MS spectra of [M − H]− ion corresponding to (C) O-desmethyl tramadol glucuronide, (D) N,O-didesmethyl tramadol glucuronide and (E) OH-O-desmethyl tramadol glucuronide, respectively. Note that unidentified nodes correspond to glucurono-conjugated endogenous metabolites.
