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Molecular network of sulfo-conjugated molecules generated under negative ion mode. (A) Overview on the C3 cluster of sulfo-conjugated molecules including tramadol-related metabolites. MS/MS spectra of [M − H]− ion corresponding to (B) O-desmethyl tramadol sulfate, (C) N,O-didesmethyl tramadolsulfate, and N,N,O-desmethyl tramadol sulfate, respectively. Note that unidentified nodes correspond to sulfo-conjugated endogenous metabolites.
