Table 1.
Analytical features of tramadol and its metabolites detected in plasma or urine of the intoxicated patient.
| Name | Metabolites | Plasma | Urine | ESI+ | ESI− | Reference | ||
|---|---|---|---|---|---|---|---|---|
| m/z (∆ppm) | tR | m/z (∆ppm) | tR | |||||
| M0 | Tramadol | Detected | Detected | 264.1963 (2) | 4.65 | / | / | [23] |
| M1 | O-desmethyl tramadol | Detected | Detected | 250.1802 (0) | 3.83 | / | / | [23] |
| M2 | N-desmethyl tramadol | Detected | Detected | 250.1802 (0) | 4.59 | / | / | [23] |
| M3 | N,N-desmethyl tramadol | Detected | Detected | 236.1639 (−3) | 3.9 | / | / | [23] |
| M4 | N,N,O-desmethyl tramadol | Not detected | Detected | 222.1485 (−2) | 3.41 | / | / | [23] |
| M5 | N,O-desmethyl tramadol | Detected | Detected | 236.1639 (−3) | 3.66 | / | / | [23] |
| M6 | OH-tramadol | Detected | Detected | 280.1905 (−1) | 3.7 | / | / | [23] |
| M7 | OH-N-desmethyl tramadol | Detected | Detected | 266.1752 (0) | 3.62 | / | / | [23] |
| M8 | OH-didesmethyl tramadol | Detected | Detected | 252.1591 (2) | 3.2 | / | / | [23] |
| M9 | Keto tramadol | Detected | Detected | 278.1749 (−1) | 3.7 | / | / | [23] |
| M13 | O-desmethyl tramadol glucuronide | Detected | Detected | 426.2113 (0) | 3.28 | 424.1967 (3) | 3.24 | [23] |
| M15 | N,O-desmethyl tramadol glucuronide | Detected | Detected | 412.1966 (0) | 3.29 | 410.1802 (−2) | 3.3 | [23] |
| M16 | OH-tramadol glucuronide | Detected | Detected | 456.2211 (−4) | 3.62 | 454.2084 (3) | 3.62 | [23] |
| M17 | OH-O-desmethyl tramadol glucuronide | Detected | Detected | 442.2056 (−4) | 3.22 | 440.1915 (0) | 3.22 | [23] |
| M18 | OH-N,N-desmethyl tramadol glucuronide | Not detected | Detected | 428.1913 (0) | 3.23 | / | / | [23] |
| M20 | O-desmethyl tramadol sulfate | Detected | Detected | 330.1372 (1) | 3.79 | 328.1207 (−2) | 3.83 | [23] |
| M21 | N,N,O-desmethyl tramadol sulfate | Detected | Detected | 302.1055 (−1) | 3.86 | 300.0902 (1) | 3.83 | [23] |
| M22 | N,O-desmethyl tramadol sulfate | Detected | Detected | 316.1217 (1) | 3.94 | 314.1065 (3) | 3.92 | [23] |
| M31 | N-oxide tramadol tramadol | Detected | Detected | 280.1906 (−2) | 4.76 | / | / | [23] |
| M32 | OH-O-desmethyl tramadol | Detected | Detected | 266.1752 (0) | 3.10 | / | / | [23] |
| M37 | Desmethyl keto tramadol N-glucuronide | Detected | Detected | / | / | 438.1749 (−5) | 4.6 | Not reported |
| M38 | Didesmethyl keto tramadol N-glucuronide | Detected | Detected | / | / | 424.1623 (2) | 4.3 | Not reported |
| M33 | O-Methyl Tramadol | Detected | Detected | 278.212 (2) | 5.03 | / | / | Not reported |
| M34 | N-Methyl Tramadol | Detected | Detected | 278.2121 (3) | 4.78 | / | / | Not reported |
| M35 | OH-Methyl Tramadol | Detected | Detected | 294.2063 (0) | 3.4 | / | / | Not reported |
| M36 | DiOH-Methyl Tramadol | Detected | Detected | 310.2016 (1) | 3.5 | / | / | Not reported |