FIG. 2.

D/H isotope fractionation during degradation of a mixture of toluene-d₈ and nonlabeled toluene in batch cultures. Representative curves from three replicates are shown for P. putida mt-2 (▴), strain TRM1 (●), D. cetonicum (▪), G. metallireducens (⧫), and T. aromatica (▾). For D. cetonicum (□) and strain TRM1 (○) D/H isotope fractionation was determined in batch cultures grown with nonlabeled toluene, and the natural abundance of deuterium was measured by GC-C-IRMS.