TABLE 1.

D/H isotope fractionation during growth of D. cetonicum, sulfate-reducing strain TRM1, and P. putida mt-2 with mixtures (50:50) of two selectively deuterated toluene species

Substrate mixture	D. cetonicum		Strain TRM1		P. putida mt-2
	b	αD	b	αD	b	αD
Toluene-d8 and toluene-d3	−0.002 ± 0.003a	1.002 ± 0.003	0.167 ± 0.219	0.857 ± 0.148	−0.016 ± 0.003	1.016 ± 0.007
Toluene-d3 and nonlabeled toluene	−1.251 ± 0.034	−3.991 ± 0.331	−1.280 ± 0.080	−3.572 ± 0.922	−4.218 ± 0.125	−0.311 ± 0.015
Toluene-d8 and toluene-d5	−0.679 ± 0.115	3,117 ± 2,947	−0.917 ± 0.336	12.005 ± 3.379	−2.696 ± 0.163	−0.590 ± 0.057
Toluene-d5 and nonlabeled toluene	−0.005 ± 0.004	1.005 ± 0.004	−0.012 ± 0.005	1.042 ± 0.042	−0.079 ± 0.041	1.088 ± 0.048
Toluene-d8 and nonlabeled toluene	−1,196 ± 0.075	−5.090 ± 1.990	−1.219 ± 0.254	−4.566 ± 4.145	−2.667 ± 0.163	−0.600 ± 0.652

^aMean ± standard deviation based on three independent growth experiments.